It has long been recognized that the LD50 measure (usually conducted using rats or mice) provides only a rough estimate of the acute toxicity of a chemical in humans. The Association of British Pharmaceutical Industry and the UK Chemical Industries Association have spoken out against the production of precise estimates of LD50 in rodents. However, the UK Home Office Report on the LD50 recommended that the LD50 should be allowed to continue. But scientific support for the test, as exemplified by recent papers in academic journals, has eroded significantly in the last few years.
Rowan, Andrew N.
"The LD50-The Beginning of the End,"
International Journal for the Study of Animal Problems: Vol. 4:
1, Article 4.
Available at: https://www.wellbeingintlstudiesrepository.org/ijsap/vol4/iss1/4