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A yearlong study was carried out in indigenous cattle of five upazila namely Khagrachari Sadar, Laxmichari, Matiranga, Mahalchari and Dighinala in Khagrachari Hill District, Bangladesh for determination of statuses of various biochemical parameters. Apparently healthy cattle (100) were selected and blood samples were collected aseptically. Biochemical analysis of carbohydrates, proteins, fats, minerals, electrolytes and enzymes were performed by using automated biochemical analyzer (Humalizer®-3000). The results showed that, the variations of parameters were not significant (P>0.05) between male and female. Although there was a slight increase of calcium 8.01±0.32mg/dl, glucose 69.21±2.31mg/dl, LDL 5.69±0.22mg/dl and uric acid 3.85±0.34mg/dl of female compared to 7.34±0.42, 68.32±1.81, 5.05±0.25 and 3.37±0.27 of the male. On the other hand, SGOT (100.14±4.86U/L) was slightly higher in male compared to the female (91.97±5.55 U/L). There was a slight variation in ALP (218.81±29.12U/L) of growing cattle compared to the calfs (293.57±42.78) and production cattle (300.88±27.24), although the variation was not significant (P>0.05).


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